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অখিল ভাৰতীয় আয়ুৰ্বিজ্ঞান প্ৰতিষ্ঠান, গুৱাহাটী

अखिल भारतीय आयुर्विज्ञान संस्थान, गुवाहाटी

All India Institute of Medical Sciences, Guwahati

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Swachh Bharat Logo
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Biochemistry
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The Department of Biochemistry commenced its services since the inception of the Institute in January 2021. The vision of the Department is to develop into a state-of-the-art facility that caters to the three-pronged approach adopted by the AIIMS institutions viz. Education, Patient Care, and Research. The Department plans to achieve this in the following manner:

1. Education

The Department is committed to nurturing students with advancements in Medical Biochemistry and Molecular Medicine, and to inculcate in them scientific curiosity, critical thinking, ethical attitudes, and practical skills. The Department has commenced MBBS teaching from January 2021, MD (Biochemistry) from January 2024, and PhD (Biochemistry) from July 2024 session onwards. The Department also provides specialized training for courses in allied specialties and envisions expanding the academic program to include interdisciplinary collaborations, and additional postgraduate courses to meet the evolving needs of the medical and scientific community. The Department also conducts Workshops and Continuing Medical Education (CMEs) on the latest advancements in the field at regular intervals.

2. Patient care

The Department is actively supporting the Institute in patient care by providing the latest laboratory facilities to aid in diagnosis, monitoring, and prognosis. Currently, the Clinical Chemistry Laboratory and the Immunoassay Laboratory of the Department provide examination of more than 100 laboratory parameters on a routine basis. The Department aims to expand the services to incorporate sophisticated biomarker testing involving molecular diagnostics, genomics, and proteomics. The Department also emphasizes providing primordial and primary screening facilities for the diseases prevalent in the community. The Department has also commenced the process of obtaining NABL accreditation for its clinical laboratory examinations.

3. Research

The Department is committed to perform health-oriented research and basic science research in the diverse fields of biomedical research such as cancer biology, cardiovascular diseases, neurobiology, metabolic diseases, molecular pharmacology, genomics, transcriptomics, medical genetics, molecular biophysics, data mining, etc. The Department carries out research in the forms of extramural funded projects, intramural research grant supported projects, MD and PhD theses, and independent faculty and student projects. The Department has also established collaborations with academic and research institutes engaged in multidisciplinary research across the country.

Current Staff
  1. Faculty - 06
  2. Senior Residents (Non-academic) - 05
  3. Junior Residents (Academic)/ MD Biochemistry students - 04
  4. PhD Scholars - 02
  5. Laboratory Technicians (Regular) - 03
  6. Laboratory Attendants (Regular) - 02
  7. Laboratory Technicians (Contractual) - 08
  8. Laboratory Attendants (Contractual) - 02
  9. Nursing Officers - 01
  10. Data Entry Operator - 01
  11. Multi-tasking Staff - 01

Faculty :

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Dr. Subash Chandra Gupta
Professor & Head of the Department
scgupta@aiimsguwahati.ac.in

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Dr. Indrajit Nath
Additional Professor
indrajitnath@aiimsguwahati.ac.in
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Dr. Jayanta Das
Additional Professor
jayantadas@aiimsguwahati.ac.in

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Dr. Ashikh Seethy
Associate Professor
ashikh.seethy@aiimsguwahati.ac.in
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Dr. Chandan Kumar Nath
Associate Professor
chandannath@aiimsguwahati.ac.in

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Dr. Amit Kumar Sonkar
Assistant Professor
amitsonkar@aiimsguwahati.ac.in

Workshop :

CMEs/ Workshops/ Other Events Organised

2025:

  1. Observance of National Science Day 2025, organised by Department of Biochemistry in collaboration with Departments of Anatomy & Physiology AIIMS Guwahati - February 28, 2025.
  2. CME on ‘DNA to Immunity: Two Pillars of Health and Disease’ on the Occasion of “DNA Day” (Date: 25th April), “International Immunology Day” (Date: 29th April), and Completion of Four Years Milestone of Biochemistry at AIIMS Guwahati - May 24, 2025.

2024:

  1. CME on Multi-Disciplinary Approach for Cancer Management on the occasion of World Cancer Day, organised by Department of Biochemistry in collaboration with Departments of Pathology & Lab Medicine, Radiodiagnosis & Interventional Radiology, Obstetrics & Gynecology, Radiation Oncology, & Medical Oncology/Hematology, AIIMS Guwahati - February 06, 2024.
  2. CME cum Workshop on Best Practices in Sample Collection, organised by Departments of Pathology and Lab Medicine, Biochemistry, Transfusion Medicine, and Microbiology – AIIMS Guwahati in collaboration with BD India Pvt Ltd. (Specimen Management Division) for celebration of the Phlebotomists Recognition Week - February 17, 2024.
  3. Observance of National Science Day 2024, organised by Department of Biochemistry in collaboration with Departments of Anatomy & Physiology AIIMS Guwahati - February 28, 2024.
  4. CME on Current Trends in OMICS - August 12, 2024.
  5. CME cum Workshop on Quality Control (QC) Analytics and Six Sigma Methodology in Lab Medicine - November 09, 2024.
  6. Pre-conference CME of ACCLMPCON-2025 - Unlocking the Secrets: Impact of Laboratory Medicine on Liver and Gastrointestinal Health' - December 14, 2024.

2023:

  1. CME on Cancer Scenario in Northeast India on the occasion of World Cancer Day, organised by Department of Biochemistry in collaboration with Departments of Pathology & Lab Medicine, Radiodiagnosis & Interventional Radiology, Obstetrics & Gynecology, Radiation Oncology, & Medical Oncology/Hematology,  AIIMS Guwahati - February 04, 2023.
  2. Observance of National Science Day 2023, organised by Department of Biochemistry, AIIMS Guwahati - February 28, 2023.
  3. Workshop on Research Grant Writing, organized by Department of Biochemistry and Research Cell – AIIMS Guwahati - March 17 2023
  4. CME cum Workshop on Best Practices in Sample Collection, organised by Departments of Biochemistry, Pathology and Lab Medicine, Transfusion Medicine, and Microbiology – AIIMS Guwahati in collaboration with BD India Pvt Ltd. (Specimen Management Division) - September 23, 2023
  5. CME on Good Laboratory Practices & Essentials of Quality Control in Clinical Chemistry, organised by Department of Biochemistry, AIIMS Guwahati - December 09, 2023
Invited Lectures:
  1. Dr. Pradeep K Srivastava (Former Dy. Director, CSIR-CDRI - Lucknow) on 'DNA Technology and Nanotechnology: Will these Change the Future of Medical Science?' - July 14, 2021.
  2. Dr. Dinesh Kumar (Associate Professor, CBMR - Lucknow) on 'Biomolecular NMR and its emerging biomedical applications' - March 30, 2024.
  3. Prof. Sudip Sen (Professor of Biochemistry, AIIMS New Delhi) on 'Stem cells: Basics and Potential Research Applications' - November 30, 2024.
  4. Prof. Ayyalusamy Ramamoorthy (Florida A&M University, Florida State University - USA) on 'Membrane Catalysed Protein-Protein Interactions Implicated in Drug Metabolism and  Amyloid Aggregation' - December 16, 2024.
  5. Dr. Ratnasekhar CH (Senior Scientist - CSIR- Central Institute of Medicinal and Aromatic Plants, Lucknow) on 'Metabolomics and lipidomics-based approach for early prediction of Parkinson's disease' - May 13, 2025.

Infrastructure :

Molecular Biology

  • Real Time PCR

  • Thermal Cycler (Gradient PCR)

  • Vertical Gel Electrophoresis Systems

  • Horizontal Gel Electrophoresis Systems

  • Chemiluminescence-based Gel-imaging System

  • Transilluminators

  • Transfer Systems for Western Blot

  • Nano-spectrophotometer

  • Flurometer

Immunology and Cell Biology

  • Flow Cytometer

  • Immunofluorescence Microscope

  • ELISA Readers

  • ELISA Plate Washers

  • Multimode Reader

  • CO₂ Incubators

  • Laminar Flow Hoods

  • BSL-2 Biosafety Cabinets

Bioinformatics and Computational Biology

  • High-end Computing Systems

Clinical Chemistry and Diagnostics

  • Fully Automated High-throughput Clinical Chemistry (Dry Chemistry) and Immunoassay Analyzers

  • Fully Automated Capillary Electrophoresis System

  • Semi-auto-analysers

  • HPLC for Hemoglobin Typing and HbA1c

  • Electrolyte Analyzer

General Laboratory Equipment

  • Refrigerated Centrifuges

  • Refrigerated Microcentrifuges

  • Orbital Shakers

  • Spectrophotometer

  • pH Meters

  • Ice Flaking Machine

  • Tissue Homogenizer

  • Lyophilizer

  • Water Purification Systems

  • Deep Freezers (-20°C and -80°C)

  • Peristaltic Pump

  • Ultrasonicator

Research and Publications :

The Department is dedicated to advancing health-oriented and basic science research across diverse biomedical fields, including cancer biology, cardiovascular diseases, neurobiology, metabolic diseases, molecular pharmacology, genomics, transcriptomics, medical genetics, molecular biophysics, and data mining. Research initiatives within the Department encompass extramurally funded projects, intramural research grants, MD and PhD thesis work, and independent projects led by faculty and students. Additionally, the Department actively collaborates with academic and research institutions nationwide, promoting multidisciplinary research to address complex biomedical challenges.

Research Projects

Ongoing/ Approved

  1. Targeting triple negative breast cancer by engineering agents derived from Piper longum; Funding Agency: ICMR, India; PI: Prof. Subash Gupta; Total Budget: INR 73,60,717.00; Period: 2024-27
  2. Taskforce on Establishment of Reference Intervals in Indian Population (TERIIP): A Multi-Centric Observational Cross Sectional Study; Funding Agency: ICMR, India; PI: Dr. Ashikh Seethy; Total Budget: Rs 1.65 Crores; Period: 2025-28
  3. Targeting Orientia tsutsugamushi in Scrub Typhus: Identifying Novel Drug Targets and Advancing Therapeutic Strategies; Funding Agency: Anusandhan National Research Foundation (ANRF), India; PI: Dr. Amit Kumar Sonkar; Total Budget: Rs. 65,94,480.00; Period: 2025-28.
  4. Taskforce on Establishment of Reference Intervals among smokers in Indian Population: A Multi-Centric Observational Cross Sectional Study; Funding Agency: ICMR, India; PI: Dr. Ashikh Seethy; Total Budget: INR 30 lakhs (tentative); Period: 2025-27
  5. Identification and characterization of stem cells phenotype in the oral cancer; Funding Agency: AIIMS Guwahati (Intramural Research Grant); PI: Prof. Subash Gupta
  6. An analysis of the immunoinflammatory status of patients with autoimmune hypothyroidism with metabolic syndrome; Funding Agency: AIIMS Guwahati (Intramural Research Grant); PI: Dr Indrajit Nath
  7. Drug related Biochemical alterations: A Biochemistry laboratory-based causality assessment study; Funding Agency: AIIMS Guwahati (Intramural Research Grant); PI: Dr Jayanta Das
  8. Biochemical and Biophysical Characterization of Alanine Racemase for Identification and Development of Potential Antimycobacterial Drugs; AIIMS Guwahati (Intramural Research Grant); PI: Dr. Amit Kumar Sonkar
  9. Performance characteristics of Glycated Haemoglobin estimation in blood samples by ion exchange chromatography using Sodium fluoride vial compared to standard EDTA anticoagulant; Funding Agency: AIIMS Guwahati (Intramural Research Grant); PI: Dr. Chandan Kumar Nath
  10. Phased globin gene sequencing to decipher genomic variants in cis in Hemoglobin E disorders; Funding Agency: AIIMS Guwahati (Intramural Research Grant); PI: Dr. Ashikh Seethy
  11. Computational analysis of the role of tumor immune micro-environment in Myelodysplastic Neoplasms (MDS) and their progression to AML-myelodysplasia related (AML-MR); Funding Agency: ICMR, India (STS Project); Mentor: Dr. Ashikh Seethy; Period: 2024-25.
  12. Investigating the role of BDNF, an indicator of neuro-synaptic functioning, as blood-based biomarker for early detection and severity prediction of dementia - A case-control study; Funding Agency: ICMR, India (STS Project); Mentor: Dr. Indrajit Nath; Period: 2024-25.
  13. Association of NFKB1 polymorphism with inflammatory markers and C-terminal agrin fragments in patients with type 2 diabetes and sarcopenia; MD thesis (Dr Ayesha Siddiqui); Guide: Dr Indrajit Nath
  14. Assessment of Clonal Hematopoiesis in Cervical Cancer and its Role in Cancer-Related Anemia; MD Thesis (Dr. Shriya Sharma); Guide: Dr. Ashikh Seethy
  15. A study on the expression of long non-coding RNAs and metabolic profile in oral cancer; MD thesis (Dr Syed Anees Fazal); Guide: Prof. Subash Gupta
  16. PTPN22 gene polymorphism in Rheumatoid Arthritis patients and its impact on disease severity; MD thesis (Dr. Md. Minjaz Zubair); Guide: Dr. Jayanta Das
  17. A study on the association of long non-coding RNAs expression with cellular metabolites in head and neck cancer; PhD thesis (Mr Arindam Dutta); Guide: Prof. Subash Gupta
  18. Identification and characterization of potential drug targets in Orientia tsutagamushi for scrub typhus; PhD thesis (Mr Lalramtluanga); Guide: Dr. Amit Kumar Sonkar

Completed

  1. Targeting long non-coding RNAs by agents derived from Moringa oleifera for breast cancer therapy; Funding Agency: ICMR, India; PI: Prof. Subash Gupta; Total Budget: INR 38,00,000.00; Period: 2020-23.
  2. An In-silico Analysis of Genetic Alterations of Human Pseudo-autosomal Regions in Acute Myeloid Leukemia; Funding Agency: ICMR, India (STS Project); Mentor: Dr. Ashikh Seethy; Period: 2022-23.
  3. Computational analysis of the role of nuclear insertions of mitochondrial origin (NUMTs) in the genesis of pathogenic variants in monogenic disorders; Funding Agency: ICMR, India (STS Project); Mentor: Dr. Ashikh Seethy; Period: 2023-24.

For information regarding research articles and research interests, refer to the webpage of individual faculty members of the Department. Google Scholar links of faculty members are given below:

  1. Prof. Subash Chandra Gupta: https://scholar.google.com/citations?user=tCzrPS0AAAAJ&hl=en
  2. Dr. Indrajit Nath: https://scholar.google.com/citations?user=yhTqNEQAAAAJ&hl=en
  3. Dr. Ashikh A Seethy: https://scholar.google.com/citations?user=6zAHBkAAAAAJ&hl=en
  4. Dr. Chandan Kumar Nath: https://scholar.google.com/citations?user=W9CwHMsAAAAJ&hl=en
  5. Dr. Amit Kumar Sonkar: https://scholar.google.com/citations?user=NFVNg24AAAAJ&hl=en

 

Clinical Services :

The Department actively supports the Institute’s patient-care services by providing advanced laboratory facilities for accurate diagnosis, monitoring, and prognosis. Currently, the Clinical Chemistry and Immunoassay Laboratories routinely offer testing for over 100 biochemical parameters. Additionally, the Department plans to expand laboratory services to include advanced biomarker assessments utilizing molecular diagnostics, genomics, and proteomics techniques. Emphasis is also placed on providing primordial and primary screening services for diseases prevalent in the community. Furthermore, the Department has initiated the process for obtaining NABL accreditation for its clinical laboratory testing to ensure quality assurance and standardization.

Total no. of investigations from April 2024 to March 2025:  3,84,766

Services Available

Clinical Chemistry

  1. Plasma glucose
  2. Glucose tolerance test (GTT)
  3. Oral GTT (DIPSI)
  4. HbA1c (HPLC)
  5. HbA1c (Enzymatic)
  6. Serum calcium
  7. Serum phosphorus
  8. Serum uric acid
  9. Serum electrolytes (sodium, potassium, chloride)
  10. Lipid profile (total cholesterol, triglycerides, LDL, HDL, Cholesterol:HDL ratio)
  11. Kidney function tests (creatinine, serum urea)
  12. Liver function tests (total bilirubin, direct bilirubin, indirect bilirubin, total protein, albumin, globulins, A/G ratio, alkaline phosphatase, AST, ALT, GGT)
  13. Creatine kinase (CK) (Total)
  14. CK-2 / CK-MB
  15. Serum magnesium
  16. Serum amylase
  17. Serum lipase
  18. Serum iron
  19. C-reactive protein (Quantitative)
  20. LDH
  21. Serum osmolality
  22. Serum lithium
  23. Transferrin saturation
  24. Direct total iron-binding capacity (dTIBC)
  25. Serum-pleural effusion albumin gradient (SPAG)
  26. Serum-ascites albumin gradient (SAAG)
  27. Serum adenosine deaminase (ADA)
  28. Rheumatoid Factor (RF)
  29. Neonatal bilirubin (NBil)
  30. Creatinine clearance
  31. Glucose-6 Phosphate Dehydrogenase (G-6-PD)
  32. Spot urine sodium
  33. Spot urine potassium
  34. Urine random calcium
  35. Urine random magnesium
  36. 24-hour urine calcium
  37. Urine random phosphorus
  38. 24-hour urine protein
  39. Spot urine chloride
  40. Urine random creatinine
  41. Urine osmolality
  42. Urine random uric acid
  43. Urine random albumin
  44. 24-hour urine creatinine clearance
  45. Urine albumin creatinine ratio (UACR)
  46. Urine protein creatinine ratio (UPCR)
  47. Fluid electrolytes (sodium, potassium, chloride)
  48. Fluid glucose
  49. Fluid LDH
  50. Fluid albumin
  51. Fluid uric acid
  52. Fluid protein
  53. Fluid amylase
  54. Fluid lipase
  55. Fluid ADA
  56. Synovial fluid analysis (glucose, protein, LDH, uric acid)
  57. Pleural fluid analysis (glucose, protein, LDH)
  58. CSF glucose
  59. CSF protein
  60. CSF ADA

Immunoassay

  1. Thyroid stimulating hormone (TSH)
  2. Free T3 (fT3)
  3. Free T4 (fT4)
  4. Total T3 (T3)
  5. Total T4 (T4)
  6. Follicle-stimulating hormone (FSH)
  7. Luteinizing hormone (LH)
  8. β-human chorionic gonadotropin (β-HCG)
  9. Total testosterone
  10. Progesterone
  11. Cortisol
  12. Insulin
  13. C-peptide
  14. Parathyroid hormone, intact (PTH)
  15. Ferritin
  16. 25-OH-vitamin D3
  17. Vitamin B12
  18. Folate
  19. Carcinoembryonic antigen (CEA)
  20. Alpha fetoprotein (AFP)
  21. Total PSA
  22. Free PSA
  23. CA 19.9
  24. CA 125
  25. NT-ProBNP
  26. Trop I
  27. Procalcitonin
  28. Prolactin
  29. CK-2 / CK-MB
  30. Serum homocysteine

Teaching & Training :

The Department is dedicated to fostering student growth through advancements in Medical Biochemistry and Molecular Medicine, cultivating scientific curiosity, critical thinking, ethical values, and practical expertise. It initiated MBBS teaching in January 2021, followed by the launch of the MD (Biochemistry) program in January 2024 and the PhD (Biochemistry) program in July 2024. Additionally, the Department offers specialized training modules for allied specialties and aims to expand its academic offerings through interdisciplinary collaborations and the introduction of further postgraduate courses, addressing the evolving demands of the medical and scientific communities. Regular workshops and Continuing Medical Education (CME) sessions are conducted to disseminate knowledge about recent developments in the field. The Department is also involved in providing training in Biochemistry to the B.Sc. (Nursing) students of the Institute.

Faculty:

Prof. Subash C Gupta Professor and Head
Dr. Indrajit Nath Additional Professor
Dr. Jayanta Das Additional Professor
Dr. Ashikh Seethy Associate Professor
Dr. Chandan Kumar Nath Associate  Professor
Dr. Amit Kumar Sonkar Assistant Professor

 

Residents:

Dr. Bidyut Bhuyan Senior Resident (Non-academic)
Dr. Lanu Mona Aimol Senior Resident (Non-academic)
Dr. Krishnarjuna Bankala Senior Resident (Non-academic)
Dr. Aroop Viswas Senior Resident (Non-academic)
Dr. Mitali Roy Senior Resident (Non-academic)
Dr. Ayesha Siddiqui Junior Resident (Academic)/ MD Student
Dr. Shriya Sharma Junior Resident (Academic)/ MD Student
Dr. Syed Anees Fazal Junior Resident (Academic)/ MD Student
Dr. Md. Minjaz Zubair Junior Resident (Academic)/ MD Student

 

PhD Scholars:

Mr. Arindam Dutta Guide: Prof. Subash C Gupta
Mr. Lalramtluanga Guide: Dr. Amit Kumar Sonkar